What is meant by solid phase synthesis?
Definition. Solid-phase synthesis is the synthesis of chemical compounds whereby the reactant molecule is chemically bound to an insoluble material and reagents are added in the solution-phase.
Which is a main advantage of solid phase synthesis?
General advantages of solid phase synthesis are easy purification, rapid generation of linear peptide intermediates, and precedent in the synthesis of large peptides.
What is solid phase peptide synthesis used for?
This method is used for the synthesis of peptides, deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and other molecules that need to be synthesised in a certain alignment. More recently, this method has also been used in combinatorial chemistry and other synthetic applications.
Which resins are used in solid phase synthesis?
The most commonly used solid support is Merrifield resin, which is a pure polystyrene (PS) polymer, cross-linked with 1–2% of divinylbenzene. Due to its low cost, high-loading properties, and chemical inertness, it is often used for solid phase synthesis of oligosaccharides.
What is solid phase reagent?
Solid-phase chemistry is a category of analytical techniques characterized by three features: dry reagents fixed on or in an inert carrier; they are transformed into a liquid state of reactivity solely by fluid from the sample, movement of fluid in the carrier happens through capillary surface forces.
What is meant by solid phase synthesis in combinatorial chemistry?
A common combinatorial technique uses organic synthesis on solid support. Linker groups are attached to resin beads in the solid phase and the reactants are passed over in solution to make the intermediates and a final detachment-from-resin reaction is run to get the desired product out of the column.
Why is Solid Phase Peptide Synthesis better than liquid phase?
With the exception of the synthesis of short peptide sequences (i.e., less than 5 amino acid residues), SPPS is faster, more efficient and economical than liquid-phase peptide synthesis (LPPS). Some of the advantages of SPPS include: Excess reagents and products can be easily washed away.
What are the benefits of solid phase peptide synthesis compared to solution phase synthesis?
In contrast, solid-phase method offers several distinct advantages over solution-phase procedure: it is simple and can be performed rapidly using a synthesizer, requires smaller amount of peptide, minimizes the purification steps and eliminates the need for solution-phase chelator conjugation since the chelator can be …
What is solid phase extraction used for?
Solid-phase extraction (SPE) is a sample preparation technique routinely used in analytical laboratories for the extraction of analytes from a complex matrix. This sample preparation technique enables the extraction, cleanup and concentration of analytes prior to their quantification.