What is goat serum used for?
Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
How do you dilute normal goat serum?
Product Specific Information For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume).
What is in goat serum?
Normal Goat Serum (10%) This 10% Normal Goat Serum blocking solution is ready-to-use, in phosphate buffered saline (pH 7.4) with 0.1% sodium azide. This normal goat serum is intended for use as a blocking solution when a second antibody of goat origin is applied in an immunohistochemical staining procedure.
How do I disable goat serum?
You can heat-inactivate by incubating the fully thawed, unopened serum bottle in a 56 degrees C water bath for 30 min. Be sure to swirl the bottle several times during the 30 min incubation.
What is a blocking solution?
The Blocking Solution prevents nonspecific binding to surfaces. By incubating the surface with The Blocking Solution (e.g. microtiter plate or western blotting membrane), free spaces on the surface are saturated by the components of The Blocking Solution.
What is donkey serum?
Donkey Serum is a sterile filtered preparation of donkey serum prepared from donkey whole blood following clotting.
Is blocking necessary for immunofluorescence?
Blocking is an important step for minimizing unspecific binding of the primary antibody within the cell. To achieve this, proteins from Bovine Serum Albumin (BSA), milk powder or serum can be used.
Why is blocking necessary?
Blocking is essential for preventing non-specific binding of antibodies or other reagents to the tissue. Even if the antibody has high specificity towards the target, intermolecular forces can promote non-specific binding to other molecules.
How do you dilute a donkey serum?
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
What is normal serum for blocking?
Normal serum at 1-5% (w/v) is a common blocking buffer component, because serum carries antibodies that bind to reactive sites and prevent the nonspecific binding of the secondary antibodies used in the assay.