What is a flow perfusion bioreactor?
Flow perfusion bioreactor: Bioreactors above are limited in ability to perfuse medium into the scaffold core. Perfusion bioreactors consist of a chamber where medium perfuses through the scaffold. Perfusion bioreactors decrease the inner and outer mass-transfer boundary layers and improve cell viability [40].
What is a scaffold bioreactor?
Bioreactors are often used in combination with scaffolds as the nutrient flow can enhance oxygen and diffusion throughout the 3D constructs to prevent the formation of necrotic cores. A variety of scaffolds have been fabricated to achieve a structural architecture that mimic native extracellular matrix.
How do you calculate bioreactor flow rate?
1) Flow rate Q=V/RT That is Flow rate (Q) in ml/min is equal to the reactor volume (V) in ml, divided by the Residence Time (RT) in minutes. It can be measured empirically in a reactor in steady state, as the volume of effluent from the reactor per unit of time.
What are the main features needed for a bioreactor intended for bioprocess engineering?
In essence, the bioreactor needs to provide the appropriate physical stimulation to cells, continuous supply of nutrients (e.g. glucose, amino acids), biochemical factors and oxygen, diffusion of chemical species to the construct interior, as well as continuous removal of by-products of cellular metabolism (e.g. lactic …
What is static cell culture?
In static cell culture, the culture medium is supplied in a batch-wise manner, and is replaced by fresh medium manually in regular intervals.
What is dynamic cell culture?
Dynamic cell culture describes the in vitro culture of cells in the presence of applied mechanical stress. Of great emerging interest is the ability to control the flow of fluid, such as medium, over cells while they grow.
What is Vvm flow rate?
As VVM stands for volume of air sparged (in aerobic cultures) per unit volume of growth medium per minute, it is calculated by dividing measured airflow rate (units: L/m, using a rotameter) with the volume (L) of growth medium (including cultured cells).
What is aeration rate?
Fermentation Design Laboratory scale work frequently reports aeration rates as the volume of air at standard conditions per volume of liquid per minute, or standard cubic feet of air per hour per gallon.
What is the difference between static and dynamic culture?
Culture is dynamic and thus complex. Culture is fluid rather than static, which means that culture changes all the time, every day, in subtle and tangible ways. Because humans communicate and express their cultural systems in a variety of ways, it can be hard to pinpoint exactly what cultural dynamics are at play.