What are morpholino used for?
Morpholinos block access of other molecules to small (~25 base) specific sequences of the base-pairing surfaces of ribonucleic acid (RNA). Morpholinos are used as research tools for reverse genetics by knocking down gene function.
What is Vivo morpholino?
Vivo-Morpholinos* are the knockdown, exon-skipping or miRNA blocking reagent of choice for in vivo experiments. A Vivo-Morpholino is comprised of a Morpholino oligo with a unique covalently linked delivery moiety, which is comprised of an octa-guanidine dendrimer.
What is a splice blocking morpholino?
Splice Blocking: By blocking sites involved in splicing pre-mRNA, Morpholinos can be used to modify and control normal splicing events. This activity can be conveniently assayed by RT-PCR, with successful splice-modification appearing as changes in the RT-PCR product band on an electrophoretic gel.
What are antisense morpholino oligonucleotides?
Morpholinos are synthetic antisense oligonucleotides (around 25 nucleotides) designed to bind and block the translation initiation complex of messenger RNA (mRNA) sequences. This technology has been used to test the role of specific genes by transient blocking, particularly during development.
What is isoform chemistry?
Definition of isoform : any of two or more functionally similar proteins that have a similar but not an identical amino acid sequence.
How do antisense oligonucleotides work?
Small pieces of DNA or RNA that can bind to specific molecules of RNA. This blocks the ability of the RNA to make a protein or work in other ways. Antisense oligonucleotides may be used to block the production of proteins needed for cell growth.
What is the difference between the sense and antisense strand?
The main difference between sense and antisense strand is that sense strand is incapable of being transcribed into mRNA whereas antisense strand serves as the template for the transcription.