How do you process a lipemic sample?
Centrifugation. A recommended procedure for treating lipemic samples is centrifugation using ultracentrifuge which effectively removes lipids and allows measurement of large number of analytes (42,43). However, due to the high cost, this equipment it is not available in a large number of laboratories.
Why is lipemic sample not accepted?
Lipemia results from sample turbidity from accumulation of lipoprotein particles and can interfere with laboratory analysis by several mechanisms. First, lipemia can increase absorption of light and thereby decrease light transmittance used for spectrophotometric analysis.
What analytes are affected by lipemia?
Conclusion: Lipemia causes clinically significant interferences for phosphorus, creatinine, total protein and calcium measurement and those interferences could be effectively removed by ultracentrifugation.
How can lipemia can be avoided?
One way to avoid grossly lipemic samples is to ask that patients fast for 12 hours before sample collection. If this is impractical, a mechanical-based means of clot detection should be available when samples are grossly lipemic.
What is lipemic or Lactescent specimen?
Lipemia (lactescence) is caused by increased triglycerides (usually as chylomicrons and infrequently from high concentrations of very low density lipoproteins).
What is lipemic?
Lipemia is a measure of serum transparency. High levels of blood lipids, mostly triglycerides, increase serum turbidity. The lipemia result is expressed in “number of plus signs,” ranging from negative to four pluses (++++).
What is the normal appearance of serum after centrifugation?
After centrifugation, what remains is a clear, straw-colored liquid on top of a dark red clot (the clumped blood cells tangled in the fibrin mesh). This straw-colored, acellular liquid is called serum (see Figure 2). FIGURE 2: Serum – the acellular fraction of blood that has been allowed to clot.
What is a lipemic or Lactescent specimen?
Is lipemic serum acceptable for chemistry testing?
Conclusion. Lipemia is a well-known interferer in clinical chemistry. One cannot avoid lipemia, but fortunately, severe lipemia is a rare phenomenon in the laboratory, and for assessment of some analytes in a lower degree of lipemia, use of serum blank eliminates the need for ultracentrifuge.
How do you remove lipemic serum?
Conclusions: High-speed centrifugation (10,000×g for 15 minutes) can be used instead of ultracentrifugation to remove lipemia in serum/plasma samples.
What is the purpose of centrifugation in blood sample?
In laboratories performing biochemical analyses on body fluids, centrifuges are routinely used to separate blood cells from serum/plasma, to separate sediment from urine, to measure the volume fraction of erythrocytes in blood (the hematocrit), and to separate bound from free components in protein binding and …
Why does lipemia in specimen cause an inaccurate hemoglobin value?
Lipemic specimens contain high levels of triglycerides consisting of chylomicrons and very low-density lipoprotein particles, which in turn cause turbidity. This turbidity interferes with light scatter and the absorption of light, resulting in a false increase of hemoglobin determinations.