What is ododn 2216?

What is ododn 2216?

ODN 2216 is a class A CpG ODN with a preference for human TLR9. Class A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string. They induce high IFN-α production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-κB signaling.

What is the storage temperature for ODN 2216?

Upon resuspension, prepare aliquots of ODN 2216 and store at -20 °C. Resuspended product is stable for 6 months at -20 °C when properly stored. Avoid repeated freeze-thaw cycles.

Can ododn 2216 be used to stimulate TLR9 in HEK-blue cells?

ODN 2216 can be used to stimulate TLR9 in HEK-Blue™ TLR9 cells. HEK-Blue™ TLR9 cells stably overexpress the TLR9 gene and an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. For more information, visit: www.invivogen.com.

What is a Class A CpG ODN?

CpG ODNs activate Toll-like receptor 9 (TLR9), leading to strong immunostimulatory effects. ODN 2216 is a Class-A CpG ODN that preferentially binds to human TLR9. Class A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string.