Is phalloidin a fluorescent?
Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 provides green fluorescence of unparalleled brightness and photostability. Alexa Fluor® 488 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations.
What is FITC phalloidin?
Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC). • Selectively stains F-actin. • Excitation/Emission: 496/516 nm. • Superior to antibody staining. • Optimal for fixed and permeabilized samples.
How does phalloidin stain work?
Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). It binds to all variants of actin filaments in many different species of animals and plants.
How does rhodamine phalloidin work?
How does rhodamine phalloidin work? The cyclic phalloidin molecule specifically binds and stabilizes the F-actin of the cellular cytoskeleton; the rhodamine acts as a marker, fluorescing in the orange-red spectrum and allowing visualization of the filamentous network within the cell.
What is the use of phalloidin?
Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments.
Is phalloidin a fluorophore?
Phalloidin, a toxin isolated from the death cap mushroom, Amanita phalloides, binds to filamentous actin with high affinity, and this has made fluorophore-conjugated phalloidin a useful tool in cellular imaging.
What is F actin staining?
Description F-Actin Stain is an easy-to-use probe-based solution for visualizing filamentous actin structures in fixed mammalian cells by fluorescence microscopy. F-actin is a major component of the cytoskeleton and is involved in fundamental cellular processes, such as cell division, morphogenesis, and migration.
Why is phalloidin toxic?
Phalloidin is one of a group of toxins from the death cap (Amanita phalloides) known as phallotoxins. It binds actin, preventing its depolymerization and poisoning the cell. Phalloidin binds specifically at the interface between F-actin subunits, locking adjacent subunits together.
Why is phalloidin used?
Phalloidin overview Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments.
What is rhodamine used for?
Rhodamine B /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers.
What is Phalloidin rhodamine?
Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC). Also available as a room-temperature-stable, ready-to-use solution: ActinRed 555 Ready Probes Reagent.
What is a fluorescein phalloidin probe?
Fluorescein phalloidin is a high-affinity F-actin probe conjugated to the green fluorescent dye, fluorescein (FITC). Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used in imaging applications to selectively label F-actin.
What is the difference between fluorescently-labeled phalloidin and fluorescein?
Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while fluorescein provides green fluorescence.
What is the difference between fluorescein phalloidin (F-actin) and DAPI?
F-actin is shown in green (Fluorescein Phalloidin) and nuclei are shown in blue (DAPI).
What is phalloidin used for?
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides ‘death cap’ mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals.